Mouse IL-10(Interleukin-10) ELISA Kit
Cat No.: EM0100 | Size: 48T & 96T
Synonyms: | Interleukin-10 ELISA Kit, IL-10 ELISA Kit, Cytokine synthesis inhibitory factor ELISA Kit, CSIF ELISA Kit, IL10 ELISA Kit |
Detection Method: | Sandwich ELISA, Double Antibody |
Application: | This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-10 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-10 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-10 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
Range: | 15.625-1000pg/ml |
Sensitivity: | 9.375pg/ml |
Species: | Mouse |
Storage: | 2-8°C(Sealed), Don't cryopreserve. |
Note: | For Research use only |
Please Note: Manuals on the web are for your reference only, specifications are subject to the delivery manuals.