Rat Cat(Catalase) ELISA Kit
Cat No.: ER0264 | Size: 48T & 96T
Synonyms: | Catalase ELISA Kit, CAT ELISA Kit |
Detection Method: | Sandwich ELISA, Double Antibody |
Application: | This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Cat antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Cat bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Cat in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
Range: | 31.25-2000mIU/ml |
Sensitivity: | 18.75mIU/ml |
Species: | Rat |
Storage: | 2-8°C(Sealed), Don't cryopreserve. |
Note: | For Research use only |
Please Note: Manuals on the web are for your reference only, specifications are subject to the delivery manuals.