Rat IL-1β(Interleukin 1 Beta) ELISA Kit
Cat No.: ER1094 | Size: 48T & 96T
Synonyms: | Interleukin-1 beta ELISA Kit, IL-1 beta ELISA Kit, Catabolin ELISA Kit, IL1B ELISA Kit, IL1F2 ELISA Kit |
Detection Method: | Sandwich ELISA, Double Antibody |
Application: | This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-1β antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-1β bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-1β in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value. |
Range: | 31.25-2000pg/ml |
Sensitivity: | 18.75pg/ml |
Species: | Rat |
Storage: | 2-8°C(Sealed), Don't cryopreserve. |
Note: | For Research use only |
Please Note: Manuals on the web are for your reference only, specifications are subject to the delivery manuals.