A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased the intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.54 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.
Source
PCR products and double-stranded DNA digested with appropriate restriction enzymes are phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA.
Size: 54 μg / 500 μl
Range: 100-3,000 base pairs
Number of Bands: 12
Concentration: 108 μg/ml
Recommended Load: 5 μl/well
Containing orange G & xylene cyanol FF as tracking dyes
Storage
Store at 25°C for 6 months
Store at 4°C for 12 months
Store at -20°C for 24 months
Ordering Information
Cat.No. | Pack Size |
SD003-R500 | 500 μl |