ECLong
Description
The principle of ECLong is based on chemiluminescence and is very convenient to detect the Horseradish peroxidase (HRP) activity in many assays such as Western blotting, Southern blotting, and Northern blotting. HRP catalyzes the chemiluminescent oxidation of cyclic diacylhydrazides such as luminol byhydrogen peroxide (H₂O₂). ECLong can enhance the luminol-dependent chemiluminescence and be wildly used to detect the present of HRP-conjugated antibodies or streptavidin which binding to antigen or nucleotide sequence respectively.
Size: 250mL x 2Quality Control
The quality of the ECLong is tested on a lot-to-lot basis to ensure consistent product quality.
Features
- Signal Duration: 12 hours
- Detection Method: X-ray film or imaging acquisition system
Suggested Antibody Dilution:
- Suggested Antibody Dilution
- Primary: 1/1,000 – 1/50,000
- Secondary: 1/50,000 – 1/250,000
Lower Detection Limit:
- Low-Picogram (10⁻¹⁴)
- High-Zeptomole (10⁻¹⁹)
Application
Western blotting
Quality Control
The quality of the ECLong is tested on a lot-to-lot basis to ensure consistent product quality.
Ordering Information
| Cat.No. | Pack Size |
| SM801-0500 | 500 ml |
