RiboIN RNase Inhibitor
Description
RiboIN™ RNase Inhibitor is a protein which specifically inhibits ribonucleases. It is used in applications such as in vitro translation, cDNA synthesis, RNA in vitro synthesis, RNA purifications, etc. RNase inhibitor is easier to use and eliminate than the vanadyl ribonucleosides. It has a high binding affinity for pancreatic-type ribonucleases such as RNase A.RiboIN™ RNase Inhibitor inhibits a broad range of RNases, including RNase A, RNase B, RNase C, but it is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H.
Size: 2500U
Concentraction: 40 U/ μl
Storage Temperature
Store at -20°C
Source
RiboIN™
RNase Inhibitor is purified by affinity chromatography which expressing
a cloned porcine liver gene from a recombinant strain of E. coli strain
containing an overproducing clone of human placenta ribonuclease
inhibitor.
Storage Buffer
20 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.5 mM EDTA, 8 mM DTT, and 50% (v/v) glycerol.
Storage Recommendations
- Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes.
- RiboINTM RNase Inhibitor requires 1 mM DTT to maintain the activity.
Quality Control
One Unit Definition
One unit is the amount of protein required to inhibit the activity of 5 ng of RNase A by 50%.
Purity
RiboIN™ RNase Inhibitor has been experimented in 12.5% SDS-PAGE electrophoresis.
It’s greater than 90% in purity. The specific activity is >80,000 units/mg.
Recommended Use
cDNA
Synthesis: 40 units/20 μl of reaction mixture, RiboIN™ RNase Inhibitor
protects mRNA and improves total cDNA yields including percent total
full length of cDNA.
RT-PCR: 40 units/20 μl of reaction mixture.
In
Vitro Transcription: 20-40 units/10 μl of reaction mixture, RiboIN™
RNase Inhibitor has been shown to be useful for the isolation of intact
RNA transcripts using T3, T7 and SP6 RNA Polymerases.
Applications
- RNA purification
- RT-PCR
- in vitro RNA transcription
- in vitro protein synthesis
- cDNA preparation by reverse transcription
- Separation and identification of specific ribonuclease activities
Ordering Information
| Cat.No. | Pack Size |
| SR001-2500 | 2500U |
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