SimplyGreen QPCR Master Mix, Rox-Free
Description
A
2X concentrated mix of Taq polymerase, dNTPs, MgCl2, fluorescent dye
(detection), and proprietary buffer components, the SimplyGreen qPCR
Master Mix, No Rox provides a convenient, reliable and robust set-up for
performing quantitative real-time analysis of DNA samples. Designed
specifically for the aforementioned niche of application, the components
of Simp lyGreen qPCR Master Mix, No Rox promise topnotch performance
with respect to sensitivity, signal-to-noise ratio and elimination of
primer dimers. Furthermore, the proprietary chemical modification of t
he DNA polymerase included in this mastermix allows for hot-start PCR,
conferring a significant reduction in non-specific PCR amplification
that is otherwise of a common occurrence with regular Taq polymerases.Based
on the fact that the qPCR instruments can vary from user to user, we
suggest that the SimplyGreen qPCR Master Mix, No Rox is compatible with
qPCR instrument: BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon
2, MiniOpticon™; Roche LightCycler® (2.0, 1.5, 480, 1536,Nano); MJ
Research Opticon™, Opticon™2, Chromo® 4; Corbett Rotor-gene® (3000,6200,
62H0, 6500, 65H0, 6600).
Size: 100 rxns
Storage: Stable for up to 1 year at -20°C
Storage Conditions
Upon
arrival, the SimplyGreen qPCR Master Mix, No Rox should be stored at
-20°C and protected from light. After each experiment, the leftover
thawed mix can be stored at 4°C if it is to be used within the next 3
months. Avoid repeated freeze-thaw cycles to retain maximum performance.
The SimplyGreen qPCR Master Mix, No Rox is stable for 1 year from the
date of shipping when stored and handled properly.
Applications
- Gene Expression (mRNA) Analysis
- Genetic Variation Analysis
- microRNA & Noncoding RNA Analysis
Ordering Information
| Cat.No. | Pack Size |
| SQ103-0100 | 100 rxns |
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